ShortReadManager at Mac App Store analyse

When you obtain data from a next-generation sequencer, you'll have a lot of reads. However, these reads contain errors at a certain rate, so caution is needed. For example, when reading a genome sequence at 100x coverage, a 31-mer that appears only once is likely considered an artifact due to some random reason, such as a sequencer reading error. Additionally, junctions between the genome sequence and adapter sequences can also occur randomly and will have low frequencies. Trimming low-frequency regions from the reads can simplify subsequent processing.